United Kingdom (UK)
Recombinant monoclonal antibody to PorA protein. Manufactured using AbAb’s Recombinant Platform with variable regions (i.e. specificity) from the hybridoma MN20B9.34.
UniProt Accession Number of Target Protein: Q51183; P0DH58
Alternative Name(s) of Target: PorA; Major outer membrane protein; outer membrane porin protein; PIA; Protein IA; Class 1 protein; serosubtype P1.4; serogroup B
Immunogen: The original antibody was generated using outer membrane protein preparations from Neisseria meningitidis as immunogen.
Specificity: This antibody is specific for the P1.4 subtype antigen (HVVVNNKVATH) of serogroup B of Neisseria meningitidis.
Application Notes: This antibody was used for the identification of PorA subtypes P1.4 using western blot. Antigenicity of the P1.4 epitope of PorA P1.7-2,4 in outer membrane vesicles and liposomes was tested by an inhibition ELISA (PMID: 14741154). This antibody is also reported to be highly bactericidal and was used as a positive control in a serum bactericidal assay (PMID: 14741154).
Antibody first published in:
Claassen et al. Production, characterization and control of a Neisseria meningitidis hexavalent class 1 outer membrane protein containing vesicle vaccine. Vaccine. 1996 Jul;14(10):1001-8. PMID:8873395
Note on publication:
Describes the generation of a vaccine which can induce bactericidal immune response in mice against five of the six wild type strains.