United Kingdom (UK)
Recombinant monoclonal antibody to PorM. Manufactured using AbAb’s Recombinant Platform with variable regions (i.e. specificity) from the llama peripheral lymphocytes Nb01.
UniProt Accession Number of Target Protein: B2RLE8
Alternative Name(s) of Target: Por secretion system protein porM/gldM; T9SS component cytoplasmic membrane protein PorM
Immunogen: The original antibody was isolated from a nanobody phage-display naïve library generated from the peripheral blood of llamas immunized with purified periplasmic domain (residues 36–516) of PorM of Porphyromonas gingivalis. The specific antibody was isolated by panning the library against periplasmic domain of PorM protein.
Specificity: This antibody binds N-terminal part comprising amino acids from 44–217 of the PorM protein of Porphyromonas gingivalis. PorM is a membrane protein that is involved in the assembly of the type IX secretion system (T9SS) in Porphyromonas gingivalis, a major bacterial pathogen that is responsible for periodontal disease in humans.
Application Notes: The binding specificity of this antibody towards N-terminal part of the PorM protein was determined using ELISA. The affinity of the antibody was found to be Kd= 1.8 ± 0.7 nM (PMID: 28471361).
Antibody first published in:
Duhoo et al. Camelid nanobodies used as crystallization chaperones for different constructs of PorM, a component of the type IX secretion system from Porphyromonas gingivalis. Acta Crystallogr F Struct Biol Commun. 2017 May 1;73(Pt 5):286-293. PMID:28471361
Note on publication:
Describes the generation of nanobodies against PorM protein of Porphyromonas gingivalis.