United Kingdom (UK)
Recombinant monoclonal antibody to Potato virus V coat protein. Manufactured using AbAb’s Recombinant Platform with variable regions (i.e. specificity) from the hybridoma scFv40 (SCR40).
UniProt Accession Number of Target Protein: Q9ICU1
Alternative Name(s) of Target: PVV; Potato virus V; CP; capsid protein; coat protein; Potato virus V capsid protein; PVXscFv40
Immunogen: The original antibody was raised against a continuous epitope (cryptotope) on disassembled coat protein of potato virus V.
Specificity: This antibody binds a continuous epitope (cryptotope) on the coat protein of Potato virus V. Potato virus V (PVV) is a member of the economically significant plant virus genus Potyvirus, family Potyviridae. The virus has flexuous filamentous particle which are approximately 760 nm in length and have a positive-sense RNA genome of ~9851-nucleotides that has a covalently linked 5′-terminal viral protein (VPg) and a 3′-terminal poly A. This strain was found to have deviated from Potato virus Y (PVY) and has subsequently been reported in potato cultivars in Andean region of South America and parts of Europe including Netherlands, Finland, Scotland, Norway and Sweden. PVV is transmitted in a non-persistent manner by aphids.
Application Notes: The binding characterization of this antibody to potato virus V coat protein was done using ELISA. This antibody does not cross react with potato virus Y and potato virus X strains. This antibody does not bind whole PVV virions in plate-trapped antigen ELISA. This antibody was capable of detecting potato virus V from infected leaf tissues in a western blot (PMID: 10648933).
Antibody first published in:
Hendy et al. Rapid production of single-chain Fv fragments in plants using a potato virus X episomal vector. J Immunol Methods. 1999 Dec 10;231(1-2):137-46. PMID:10648933
Note on publication:
Describes the use of PVX vector for the transient expression of an scFv antibody against coat protein of Potato virus V.