United Kingdom (UK)
Recombinant monoclonal antibody to Profilin-1 & Profilin-2. Manufactured using AbAb’s Recombinant Platform with variable regions (i.e. specificity) from the hybridoma mAbPRF12a (2-11-H7).
UniProt Accession Number of Target Protein: Q42449; Q42418
Alternative Name(s) of Target: Ara t 8; PRO1; Profilin-1; Profilin 1; Profilin; 2-11-H7; PRF1; 2-11H7; Profilin 2; PRO2; Profilin 3
Immunogen: This antibody was raised by immunising mice with a purified plant Profilin-1.
Specificity: This antibody recognizes Profilin-1, Profilin-2 and has a weak binding to Profilin-3. Profilins are actin-binding proteins involved in the processing of actin cytoskeleton. They are also recognized allergens.
Application Notes: mAbPRF12a antibody is recommended for the analysis and detection of Profilin-1. It strongly identifies Profilin-1 (and Profilin-2) via Western blot (Kandasamy et al., 2002). This antibody was used in immunohistochemical and immunofluorescence analyses to identify parts of the plant where profilins are most prevalent in (Kandasamy et al., 2002).
Antibody first published in:
Kandasamy et al. Plant profilin isovariants are distinctly regulated in vegetative and reproductive tissues. Cell Motil Cytoskeleton. 2002 May;52(1):22-32. PMID:11977080
Note on publication:
This article describes the generation and characterization of the antibody.