United Kingdom (UK)
Recombinant monoclonal antibody to Rotavirus A capsid glycoprotein VP7. Manufactured using AbAb’s Recombinant Platform with variable regions (i.e. specificity) from the hybridoma 4F8.
UniProt Accession Number of Target Protein: P12476
Alternative Name(s) of Target: VP7; RRV VP7; RRV capsid protein; outer capsid glycoprotein; capsid protein; rhesus rotavirus; RRV
Immunogen: The original antibody was generated by immunizing mice with partially purified RRV.
Specificity: This antibody binds the outer capsid glycoprotein VP7 of the Rotavirus A serotype 3. This antibody can bind rhesus and equine strains of Rotaviruses. Rotavirus is a genus of double-stranded RNA viruses in the family Reoviridae. A rotavirus infection is a contagious disease in which inflammation of the stomach and intestines occurs causing severe, watery diarrhea and vomiting in infants and young children. Nearly every child develops a rotavirus infection before the age of five.
Application Notes: This antibody neutralized serotype 3 rotaviruses RRV and simian rotavirus (SA11) in plaque reduction neutralization assay and protected suckling mice in vivo against serotype 3 (RRV and SA11) but not against OSU or UK virus challenge (Offit et al., 1986; PMID: 2422398). This antibody was used to detect rotaviruses from stools of infants with gastroenteritis using ELISA (Padilla-Noriega et al., 1990; PMID: 2166073). The crystal structure of the Fab fragment of the antibody in complex with rhesus rotavirus (RRV, serotype G3) VP7 trimer was determined. The Fab fragment showed a stronger neutralization activity against RRV than the original format (Aoki et al., 2009; PMID: 19520960).
Antibody first published in:
Shaw et al. Antigenic mapping of the surface proteins of rhesus rotavirus. Virology. 1986 Dec;155(2):434-51. PMID:2431540
Note on publication:
Describes the use of antibodies to map the functional topography of the surface proteins of rhesus rotavirus (RRV) that mediate viral neutralization.