Anti-Rpp30 [6E12]

Name: Anti-Rpp30 [6E12]
SKU: Ab03410
Availability: InStock

Description:

Recombinant monoclonal antibody to Rpp30. Manufactured using AbAb’s Recombinant Platform with variable regions (i.e. specificity) from the chicken B-cell clone 6E12.

Clone Name:

6E12

Published Application(s):

ELISA

Published Species Reactivity:

Human

Products based on this clone:

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Ab03410-23.0 Anti-Rpp30 [6E12]
Human
Rabbit IgG
Purified
In Stock

Ab03410-30.11 Anti-Rpp30 [6E12]
Human
scFv fragment (His)
ScFv
Purified
Ships in 5-6 weeks

UniProt Accession Number of Target Protein: P78346

Alternative Name(s) of Target: Ribonuclease P protein subunit p30; RNaseP protein p30; RNase P subunit 2
Immunogen: The original antibody was generated by immunizing a barnevelder chicken with an antibody antigen cocktail comprising recombinant purified human antigens α-fodrin, hPop1, HspB3, Ro52, Rpp30, Rrp4 and Rrp42 in complete Freund's adjuvant. B cells that were harvested from the bone marrow and peripheral blood lymphocytes of immunized chickens was used to generate recombinant scFv phage display libraries. The specific clone was identified by panning the library against specific antigen.

Specificity: The antibody is specific for Rpp30.

Application Notes: The specificity of the original format of the antibody was confirmed by ELISA analysis (Hof et al., 2008; PMID: 18062792).

Antibody first published in: Hof et al. Multiple-antigen immunization of chickens facilitates the generation of recombinant antibodies to autoantigens Clin Exp Immunol. 2008 Feb;151(2):367-77. PMID:18062792
Note on publication: Describes the generation of chicked antibodies against various human autoantigens.

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