United Kingdom (UK)
Recombinant monoclonal antibody to S protein. Manufactured using AbAb’s Recombinant Platform with variable regions (i.e. specificity) from the phage display antibody Fab58.
UniProt Accession Number of Target Protein: P59594
Alternative Name(s) of Target: Spike glycoprotein, Spike protein; S protein; SARS-CoV S protein; S glycoprotein; E2; Peplomer protein
Immunogen: A naive antibody library was constructed from convalescent SARS patient lymphocyte using phage display technology. The original antibody was then isolated by panning the library on purified SARS virions.
Specificity: The antibody is specific for the SARS-CoV spike protein.
Application Notes: The specificity of this antibody (Fab) was confirmed in ELISA analysis. The antibody detected the SARS-CoV S protein by western blot analysis. The clone was converted into human IgG1 and its neutralizing activity was determined. Both Fab and IgG1 antibodies showed significant neutralizing activities in cytopathic effect (CPE) inhibition neutralization test, the antibodies were able to completely neutralize the SARS virus and protect the Vero cells from CPE after virus infection (Liang et al, 2005; PMID:16544518).
Antibody first published in:
Liang et al. SARS patients-derived human recombinant antibodies to S and M proteins efficiently neutralize SARS-coronavirus infectivity. Biomed Environ Sci. 2005 Dec;18(6):363-74. PMID:16544518
Note on publication:
The paper describes the generation and characterization of the antibody.