United Kingdom (UK)
Recombinant monoclonal antibody to S100A5. Manufactured using AbAb’s Recombinant Platform with variable regions (i.e. specificity) from the hybridoma N176A/35.1.
UniProt Accession Number of Target Protein: P63083
Alternative Name(s) of Target: N176A/35.1R; N176/35;
Protein S100-A5; Protein S-100D; S100 calcium-binding protein A5
Immunogen: This antibody was raised by immunising BALB/c mice with a fusion protein amino acids 1-93 (full-length) of rat S100A.
Specificity: This antibody is specific for the S100a5 protein and does not show cross reactivity for either S100A4 or S100A6. S100a5 Binds calcium, zinc and copper. One subunit can simultaneously bind 2 calcium ions or 2 copper ions plus 1 zinc ion. Calcium and copper
Application Notes: This antibody is recommended for the detection and analysis of S100A5 by western blot and immunocytochemistry. For instance, the mouse version of this antibody was used to detect S100A5 by western blot in adult rat brain membranes and whole brain homogenates from S100A5-tauGFP (targeted deletion of S100A5 and replaced with coding region of tau fused to GFP) heterozygote and KO mice.
Antibody first published in:
Andrews et al. A toolbox of IgG subclass-switched recombinant monoclonal antibodies for enhanced multiplex immunolabeling of brain eLife. 2019; 8: e43322. PMID:30667360
Note on publication:
This article describes the generation of a library of recombinant monoclonal antibodies (R-mAbs) from a pool of mAb-producing hybridomas for neuroscience research.