United Kingdom (UK)
Recombinant monoclonal antibody to SIV-NP. Manufactured using AbAb’s Recombinant Platform with variable regions (i.e. specificity) from the phage display antibody sdAB5.
UniProt Accession Number of Target Protein: P16985
Alternative Name(s) of Target: NP; nucleoprotein; Nucleocapsid protein; Protein N
Immunogen: A VHH library was constructed from peripheral blood mononuclear cells (PBMCs) of an immunized Bactrian camel with SIV-NP recombinant protein, and the antibody was isolated by panning against SIV-NP.
Specificity: The antibody is specific for SIV-NP protein. The antibody does not cross-react with PCV2, PEDV, PRRSV, PRV, and CSFV.
Application Notes: The specificity of the original format of the antibody was confirmed by ELISA analysis. The antibody detected the SIV-NP by western blot analysis. A blocking ELISA based on the biotinylated antibody was developed for SIV detection (Du et al, 2019; PMID: 31819435).
Antibody first published in:
Du et al. Biotinylated Single-Domain Antibody-Based Blocking ELISA for Detection of Antibodies Against Swine Influenza Virus Int J Nanomedicine. 2019 Nov 29;14:9337-9349. PMID:31819435
Note on publication:
The original paper describes the generation and characterization of the antibody.