United Kingdom (UK)
Recombinant monoclonal antibody to Serum Albumin. Manufactured using AbAb’s Recombinant Platform with variable regions (i.e. specificity) from the synthetic clone Nb.b201.
UniProt Accession Number of Target Protein: P02768
Alternative Name(s) of Target: Human Serum Albumin; HSA; albumin
Immunogen: The original antibody was isolated from a synthetic nanobody library starting from a consensus framework derived from llama genes IGHV1S1–S5.
Specificity: This antibody binds a convex protrusion on the surface of human serum albumin.
Application Notes: This antibody specifically binds human serum albumin and does not cross react with mouse serum albumin. The binding specificity of this antibody towards fluorescently labeled HSA was done using flow cytometry. The binding affinity of this antibody for human serum albumin was measure by surface plasmon resonance and was found to be 430 nM (PMID: ).
Antibody first published in:
McMahon et al. Yeast surface display platform for rapid discovery of conformationally selective nanobodies. Nat Struct Mol Biol. 2018 Mar;25(3):289-296. PMID:29434346
Note on publication:
Describes the use of a yeast surface display platform for rapid discovery of conformationally selective nanobodies.