United Kingdom (UK)
Recombinant monoclonal antibody to TIGIT. Manufactured using AbAb’s Recombinant Platform with variable regions (i.e. specificity) from the hybridoma MT251.
UniProt Accession Number of Target Protein: Q495A1
Alternative Name(s) of Target: VSTM3; VSIG9; WUCAM; T-cell immunoreceptor with Ig and ITIM domains; V-set and immunoglobulin domain-containing protein 9; V-set and transmembrane domain-containing protein 3
Immunogen: The original antibody was generated by immunizing BALB/c mice with recombinant human TIGIT protein.
Specificity: The original antibody is specific for TIGIT. The antibody does not cross-react with LAG-3, PD-1 and PD-L1.
Application Notes: The original mouse antibody was subjected to humanized modification, keeping a similar binding activity. The specificity of the original and the humanized format of the antibody was confirmed by ELISA analysis (EC50= 0.09901 μg/mL and 0.07412 μg/mL respectively). The original format of antibody blocked the binding activity of TIGIT and CD155. The original format and the humanized version of the antibody could recognize the TIGIT antigen expressed by Jurkat/TIGIT cells by FACS analysis. The original format of the antibody was able to effectively block the binding of TIGIT to CD155 (CN112794909).
Antibody first published in: PMID: