Anti-TRAAK [13E9]

Description:

Recombinant monoclonal antibody to TRAAK. Manufactured using AbAb’s Recombinant Platform with variable regions (i.e. specificity) from the hybridoma 13E9.

Clone Name:

13E9

Published Application(s):

WB, ELISA

Published Species Reactivity:

Human

Products based on this clone:

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Ab03283-23.0 Anti-TRAAK [13E9]
Human
Rabbit IgG
Purified
In Stock

Ab03283-1.1 Anti-TRAAK [13E9]
Human
Mouse IgG1
Purified
In Stock

UniProt Accession Number of Target Protein: Q9NYG8

Alternative Name(s) of Target: KCNK4; TRAAK; Potassium channel subfamily K member 4; TWIK-related arachidonic acid-stimulated potassium channel protein; Two pore potassium channel KT4.1; Two pore K(+) channel KT4.1
Immunogen: The original antibody was generated by immunizing mice with recombinant human TRAAK.

Specificity: This antibody binds human TRAAK, which is a voltage-insensitive potassium channel

Application Notes: The binding characterization of this antibody was done using ELISA and western blot (PMID: 23341632).

Antibody first published in: Brohawn et al. Domain-swapped chain connectivity and gated membrane access in a Fab-mediated crystal of the human TRAAK K+ channel. Proc Natl Acad Sci U S A. 2013 Feb 5; 110(6): 2129–2134. PMID:23341632
Note on publication: Describes the antigen-antibody interactions using X-ray crystallography.

Immunofluorescence staining of HepG2 cells using Ab03283-23.0. Click on image for more details.

Immunofluorescence staining of HepG2 cells with anti-TRAAK antibody 13E9 (Ab03283). Immunofluorescence analysis of paraformaldehyde fixed HepG2 cells on Shi-fix™ coverslips stained with the chimeric rabbit IgG version of 13E9 (Ab03283-23.0) (1:100 dilution) for 1h followed by Alexa Fluor® 488 secondary antibody (1:1000 dilution), showing cytoplasmic staining. The nuclear stain is DAPI (blue). Panels show, from left-right, top-bottom, Ab03283-23.0, DAPI, merged channels, and an isotype control. The isotype control was an unknown specificity antibody (Ab00178-23.0) followed by staining with Alexa Fluor® 488 secondary antibody.

Western blot of Kelly cells lysate using Ab03283-23.0. Click on image for more details.

Western blot using anti-TRAAK antibody 13E9 (Ab03283). Kelly cells lysate (35µg protein in RIPA buffer) was resolved via SDS-PAGE, and the subsequent blot was probed with the chimeric rabbit version of 13E9 (Ab03283-23.0) at 1µg/ml before detection using an anti-rabbit secondary antibody. A primary incubation of 1 hour was used, and proteins were detected by chemiluminescence.

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