United Kingdom (UK)
Recombinant monoclonal antibody to TWEAK. Manufactured using AbAb’s Recombinant Platform with variable regions (i.e. specificity) from the hybridoma MTW-1.
UniProt Accession Number of Target Protein: O54907
Alternative Name(s) of Target: CD255; TNFSF12; TNF-related weak inducer of apoptosis; MTW1; MTW 1; Tumor necrosis factor ligand superfamily member 12;
Immunogen: This antibody was raised by immunising SD rats with CD8-mTWEAK, a fusion of the human CD8alpha extracellular region to murine TWEAK.
Specificity: This antibody is specific for murine TWEAK. It has been shown not to react with TNF-α, FasL, and TRAIL.
Application Notes: The specificity of this antibody has been confirmed in flow cytometric analysis, where it reacts specifically with mTWEAK-transfected L5178Y cells, and not with parental L5178Y cells or cells transfected with related TNF family members (Nakayama et al, 2003). Additionally, this antibody has been used to assess TWEAK cell surface expression in flow cytometric analysis (Nakayama et al, 2003). This antibody has been shown to block CD8-mTWEAK cytotoxicity against L5178Y cells over-expressing murine Fn14, the TWEAK receptor (Nakayama et al, 2003). When administered to collagen-induced arthritis (CIA) mice, this blocking antibody has been shown to have anti-inflammatory and antiangiogenic effects, with associated improvements to both the clinical and histological manifestations of CIA (Kamata et al, 2006). Additionally, administration of this antibody to denervated mice attenuates denervation-induced skeletal muscle loss (Mittal et al, 2010). Finally, administration of this antibody to CD163-/- mice subject to hind limb ischaemia inhibits the increased perfusion resulting from CD163 deficiency (Akahori et al, 2015).
Antibody first published in:
Nakayama et al. Characterization of murine TWEAK and its receptor (Fn14) by monoclonal antibodies. Biochem Biophys Res Commun. 2003 Jul 11;306(4):819-25. PMID:12821115
Note on publication:
Describes the original generation of this antibody.
Immunofluorescence staining of mouse splenocytes using anti-TWEAK antibody (Ab01385) MTW-1. Immunofluorescence analysis of paraformaldehyde fixed mouse splenocytes immobilized on Shi-fix™ cover-slips and stained with the chimeric rabbit IgG version of MTW-1 (Ab01385-23.0) at 10 µg/ml followed by Alexa Fluor® 488 secondary antibody (2 µg/ml), showing membrane staining in subset of cells. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom Ab01385-23.0, DAPI, merged channels and an isotype control. The isotype control was stained with anti-Fluorescein antibody (Ab00102-23.0) followed by Alexa Fluor® 488 secondary antibody.