Anti-Tau [MN423]

Description:

Recombinant monoclonal antibody to Tau. Manufactured using AbAb’s Recombinant Platform with variable regions (i.e. specificity) from the hybridoma MN423.

Clone Name:

MN423

Published Application(s):

ICC, WB, ELISA, IF, IHC

Published Species Reactivity:

Human

Products based on this clone:

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Ab02389-1.1 Anti-Tau [MN423]
Human
Mouse IgG1
Purified
In Stock

Ab02389-10.0 Anti-Tau [MN423]
Human
Human IgG1
Purified
Ships in 4-5 weeks

Ab02389-10.3 Anti-Tau [MN423]
Human
Human IgG1
Fc Silent™
Purified
Ships in 4-5 weeks

Ab02389-23.0 Anti-Tau [MN423]
Human
Rabbit IgG
Purified
In Stock

Ab02389-3.3 Anti-Tau [MN423]
Human
Mouse IgG2b
Fc Silent™
Purified
In Stock

Ab02389-3.0 Anti-Tau [MN423]
Human
Mouse IgG2b
Purified
Ships in 4-5 weeks

UniProt Accession Number of Target Protein: P10636

Alternative Name(s) of Target: MAPT; TAU; PHF-tau; MAPTL; MTBT1; TAU; PHF; Tau proteins; Alzheimer paired helical filaments; Paired helical filament-tau; Microtubule-associated protein tau; Neurofibrillary tangle protein; mAb 423; NOAL 6.423; NOAL 6/66.423.2
Immunogen: The original antibody was generated by hyper-immunizing C3H mice with tangle-enriched fragments of the Alzheimer paired helical filaments core.

Specificity: This antibody recognizes the pronase-resistant core PHF and the 12 kDa tau fragment but does not recognize normal full-length tau. It is also reported that this antibody speciﬁcally recognizes tau truncated at Glu391 on the C terminus side. The addition or removal of a single residue at the C-terminus abolishes immunoreactivity.

Application Notes: This antibody was characterized in detail (Novak et al., 1989; PMID: 2602435) and has been extensively utilized in Alzheimer's disease research: It played a crucial role in the structural characterization of the Alzheimer paired helical filament (PHF) core (Wischik et al., 1988; PMID: 2455299), and was employed for extracting two peptide fragments (9.5 and 12 kDa) of the PFH pronase-resistant core through immunoblotting (Wischik et al. 1988; PMID: 3132715). Additionally, it was used to study the folding of the Alzheimer’s core PHF subunit through competitive ELISA and western blotting (Skrabana et al., 2004; PMID: 15196943). It has been instrumental in the molecular investigation of neurofibrillary degeneration in Alzheimer's disease (Bondareff et al., 1994; PMID: 7509849). Epitope mapping revealed that it requires a glycine at position -3 with respect to the C terminus (Khuebachova et al., 2002; PMID: 11983234). In the realm of tau protein studies related to Alzheimer's disease, it has been employed for immunohistochemistry on brain tissue samples (Mondragón-Rodríguez et al., 2014; PMID: 24033439). Double and triple-labeled immunofluorescence was conducted using this antibody on brain tissue from Alzheimer's disease patients (Mondragón-Rodríguez et al., 2014; PMID: 24033439) (García-Sierra et al., 2003; PMID: 12719624). This antibody was used to immunoblot purified candidate tau analogs (Novak et al., 1993; PMID: 7679073). Furthermore, it was employed in immunohistochemical staining of brain tissue from patients with Alzheimer's disease, NCI, PSP, or CBD (Berry et al., 2004; PMID: 15475684). Western blot analysis on brain tissue from Alzheimer's patients was performed (Fitzpatrick et al., 2017; PMID: 28678775). This antibody was also used for a competitive ELISA on the Alzheimer's disease expression cDNA library made from the hippocampus of a 70-year-old patient who died from Alzheimer’s disease (Khuebachova et al., 2002; PMID: 11983234).

Antibody first published in: Wischik et al. Isolation of a fragment of tau derived from the core of the paired helical filament of Alzheimer disease. Proc Natl Acad Sci U S A. (1988); 85(12):4506-10. PMID:3132715
Note on publication: The original publication describes the generation and use of this antibody for the extraction of two peptide fragments of Alzheimer paired helical filaments.

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