United Kingdom (UK)
Recombinant monoclonal antibody to Tetanus toxoid. Manufactured using AbAb’s Recombinant Platform with variable regions (i.e. specificity) from the human B cell clone 9F12.
UniProt Accession Number of Target Protein: P04958
Alternative Name(s) of Target: TT; TTx; Tetanus toxin; tetX; Tentoxylysin
Immunogen: The original antibody was generated from the peripheral blood mononuclear cells form human donors after immunization with tetanus toxoids and fusing these lymphocytes with mouse myeloma line P3-X63-Ag 8.653.
Specificity: This antibody binds the intact or wild type tetanus toxin of Clostridium tetani. It was also reported to bind the tetanus toxin fragment B. Tetanus toxin is an extremely potent neurotoxin produced by Clostridium tetani.
Application Notes: The screening of hybridoma supernatants to identify binding towards tetanus toxin was done using ELISA. The protective capacity of this antibody against tetanus toxoid was tested using the mouse tetanus toxin neutralization assay. The mice which received the tetanus toxin preincubated with 9F12 were completely protected (PMID: 7174795). The original antibody was used to study the role of gangliosides in the binding and internalization of bacterial toxins (PMID: 11093135).
Antibody first published in:
Gigliotti et al. Protective human hybridoma antibody to tetanus toxin. J Clin Invest. 1982 Dec;70(6):1306-9. PMID:7174795
Note on publication:
Describes the generation and neutralization capacity of this antibody towards tetanus toxin.