United Kingdom (UK)
Recombinant monoclonal antibody to U1A. Manufactured using AbAb’s Recombinant Platform with variable regions (i.e. specificity) from the chicken B-cell clone U11B3.
UniProt Accession Number of Target Protein: P09012
Alternative Name(s) of Target: U1 small nuclear ribonucleoprotein A; U1 snRNP A; U1-A
Immunogen: The original antibody was generated by immunizing a barnevelder chicken with an antibody antigen cocktail comprising recombinant purified human antigens annexin XI, CENP-B, Topo, Jo-1, Ro60, Rpp38 and U1A in complete Freund's adjuvant. B cells that were harvested from the bone marrow and peripheral blood lymphocytes of immunized chickens was used to generate recombinant scFv phage display libraries. The specific clone was identified by panning the library against specific antigen.
Specificity: The antibody is specific for U1A. U1A is a component of the spliceosomal U1 snRNP, which is essential for recognition of the pre-mRNA 5' splice-site and the subsequent assembly of the spliceosome. U1 snRNP is the first snRNP to interact with pre-mRNA.
Application Notes: The specificity of the original format of the antibody was confirmed by ELISA analysis. The antibody detected U1A by western blot analysis (Hof et al., 2008; PMID: 18062792).
Antibody first published in:
Hof et al. Multiple-antigen immunization of chickens facilitates the generation of recombinant antibodies to autoantigens Clin Exp Immunol. 2008 Feb;151(2):367-77. PMID:18062792
Note on publication:
Describes the generation of chicked antibodies against various human autoantigens.