United Kingdom (UK)
Recombinant monoclonal antibody to VGlut1. Manufactured using AbAb’s Recombinant Platform with variable regions (i.e. specificity) from the hybridoma N28/9.
UniProt Accession Number of Target Protein: Q62634
Alternative Name(s) of Target: N28/9R; Vesicular glutamate transporter 1; Slc17a7; Brain-specific Na(+)-dependent inorganic phosphate cotransporter; Solute carrier family 17 member 7
Immunogen: This antibody was raised by immunising BALB/c mice with a fusion protein amino acids 493-560 (cytoplasmic C-terminus) of rat VGlut1
Specificity: This antibody is specific for the Vglut1 protein. The antibody does not cross-react with VGlut2 or VGlut3. Vglut1 mediates the uptake of glutamate into synaptic vesicles at presynaptic nerve terminals of excitatory neural cells. May also mediate the transport of inorganic phosphate.
Application Notes: Immunofluorescense was preformed on the brain of mice and rats using this antibody (Lindhoff et al, 2009; PMID:19285470) (Garcia-Pino et al, 2009; PMID:20151361) (Takahashi et al, 2011; PMID:21262467). This antibody has been used for immunohistochemistry on the brain of a rat (Larsson et al, 2011; PMID:21810784). The antibody was used in a western blot experiment on rat brain extracts (Garcia-Pino et al, 2009; PMID:20151361). Immunocytochemistry has been preformed on normal rat retinas with this antibody (Caminos et al, 2015; PMID:25499209).
Antibody first published in:
Andrews et al. A toolbox of IgG subclass-switched recombinant monoclonal antibodies for enhanced multiplex immunolabeling of brain eLife. 2019; 8: e43322. PMID:30667360
Note on publication:
This article describes the generation of a library of recombinant monoclonal antibodies (R-mAbs) from a pool of mAb-producing hybridomas for neuroscience research.