United Kingdom (UK)
Recombinant monoclonal antibody to VP1694. Manufactured using AbAb’s Recombinant Platform with variable regions (i.e. specificity) from the phage display antibody FA7.
UniProt Accession Number of Target Protein: Q87P24
Alternative Name(s) of Target: Type III export protein YscF
Immunogen: This antibody was generated by panning a scFv library from BALB/c mice immunized with purified fusion protein against GST-VP1694 antigen.
Specificity: This antibody is specific for the VP1694 protein from Vibrio parahaemolyticus. This proteins is necessary for this bacteria to act as a pathogen. Vibrio parahaemolyticus can cause gastroenteritis in humans.
Application Notes: An ELISA was performed on associated V. parahaemolyticus proteins (TDH, TLH, VopQ, VP1694), Homologous protein HrpA, and PBS using the scFv version of this antibody. It showed a stronger binding to VP1694 than to other proteins. Furthermore, a western blot was performed on VP1694 using this antibody. The western blot showed the binding of VP1694 by the antibody (Wang et al, 2014; pmid:25320414).
Antibody first published in:
Wang et al. Generation and Characterization of a scFv Antibody Against T3SS Needle of Vibrio parahaemolyticus. Indian J Microbiol. 2014 Jun; 54(2): 143–150. PMID:25320414
Note on publication:
A phage single-chain fragment variable antibody (scFv) library was constructed, and a specific scFv against VP1694 named scFv-FA7 was screened by phage display panning.