United Kingdom (UK)
Recombinant monoclonal antibody to Vaccinia virus. Manufactured using AbAb’s Recombinant Platform with variable regions (i.e. specificity) from the llama peripheral lymphocytes G.
Alternative Name(s) of Target: Smallpox virus
Immunogen: The original antibody was isolated from a non-immune semisynthetic llama library constructed from blood samples (6 ml) collected from the left jugular vein of three research naïve mature llamas.
Specificity: This antibody is capable of detecting live vaccinia virus in a sample.
Application Notes: The binding specificity of this antibody towards vaccinia virus was checked using ELISA. It was reported that the original VHH antibody G retained ∼40% of its activity after heating for 40min decreasing to around 3% of original activity after 80min (PMID:17165813).
Antibody first published in:
Goldman et al. Facile Generation of Heat Stable Antiviral and Antitoxin Single Domain Antibodies from a Semi-synthetic Llama Library. Anal Chem. 2006 Dec 15; 78(24): 8245–8255. PMID:17165813
Note on publication:
A semisynthetic llama library was constructed from non-immune llamas and binders specific for live vaccinia virus (a smallpox virus surrogate), hen egg lysozyme, cholera toxin, ricin, and staphylococcal enterotoxin B were selected.