United Kingdom (UK)
Recombinant monoclonal antibody to XCR1. Manufactured using AbAb’s Recombinant Platform with variable regions (i.e. specificity) from the hybridoma 5G7.
UniProt Accession Number of Target Protein: P46094
Alternative Name(s) of Target: CCXCR1; GPR5; chemokine XC receptor 1; G-protein coupled receptor 5; lymphotactin receptor
Immunogen: The original antibody was generated by immunizing XCR1 knockout mice with human XCR1-expressing B300.19 cells mixed with equal volume of GERBU adjuvant.
Specificity: This antibody binds human XCR1, more particularly it strongly binds amino acids 'PESTTFFYYDLQ' of the human XCR1. XRC1 is a receptor for chemokines SCYC1 and SCYC2 and expressed on 80% of CD8+ the dendritic cells. It also acts as a receptor for XCL1/Lymphotactin. It transduces a signal by increasing the intracellular calcium ions level.
Application Notes: This antibody is capable of binding human XCR1-EGFP-expressing B300.19 cells in a flow cytometry analysis. The determination of the binding epitope of this antibody was done using ELISA (US9371389). This antibody exhibits a significant effect in treating rheumatoid arthritis in the experiment of delayed-type hypersensitivity reaction induced by Mycobacterium butyricum delayed-type hypersensitivity reaction. The neutralizing activity of this antibody against human lymphotactin-induced migration of human XCR1-expressing cells was evaluated by in vitro chemotaxis assay. It was reported that this antibody completely inhibited cell migration at a concentration of about 3 μg/mL (US9371389). The effect of this antibody on ear swelling of DNFB (Dinitrofluorobenzene)-sensitized mice was done and it was reported that this antibody caused significant suppression of ear swelling 24 after induction. This antibody was also reported to show showed a very low reactivity to human CX3CR1-EGFP-expressing B300.19 cells (US9371389). The original antibody with saporin-conjugated secondary antibody showed growth suppression of human XCR1-EGFP-expressing B300.19 cells. The humanized version of this antibody is also available on request (US9371389).
Antibody first published in: PMID:
Immunofluorescence staining of K562 cells with anti-XCR1 antibody 5G7 (Ab03121). Immunofluorescence analysis of paraformaldehyde fixed K562 cells immobilized on Shi-fix™ coverslips stained with the chimeric rabbit IgG version of 5G7 (Ab03121-23.0) (1:100 dilution) for 1h followed by Alexa Fluor® 488 secondary antibody (1:1000 dilution), showing membrane and cytoplasmic staining. The nuclear stain is DAPI (blue). Panels show, from left-right, top-bottom, Ab03121-23.0, DAPI, merged channels, and an isotype control. The isotype control was an unknown specificity antibody (Ab00178-23.0) followed by staining with Alexa Fluor® 488 secondary antibody.