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- Ab01306-1.1 Anti-XRCC2 [7B7/3]
- Human
- Mouse IgG1
- Purified
- In Stock
- Ab01306-1.6 Anti-XRCC2 [7B7/3]
- Human
- Mouse Fab fragment
- His-Tagged
- Purified
- Ships in 4-5 weeks
- Ab01306-23.0 Anti-XRCC2 [7B7/3]
- Human
- Rabbit IgG
- Purified
- In Stock
Recombinant monoclonal antibody to XRCC2. Manufactured using AbAb’s Recombinant Platform with variable regions (i.e. specificity) from the hybridoma 7B7/3.
UniProt Accession Number of Target Protein: O43543
Alternative Name(s) of Target: XRay Repair Cross Complementing 2; XRay Repair Cross Complementing Protein 2; DNA repair protein XRCC2; X-ray repair complementing defective repair in Chinese hamster cells 2; XRCC2 7B7; 7B7
Immunogen: This antibody was raised by immunising mice with the denaturated recombinant human XRCC2 protein which had been previously amplified and purified from E. coli.
Specificity: This antibody is specific to human XRCC2 (it does not bind to other RAD51 paralogs). XRCC2 is a paralog of RAD51 which is a homologue of RecA (E. coli). It is engaged in homologous recombination repair (HRR) pathway of double-stranded DNA of chromosomal fragmentations and deletions.
Application Notes: WB: XRCC2 7B7/3 (7B7) antibody was used to visualise XRCC2 on Western blots in multiple experiments (e.g. after previous gel filtration of infected pf9 cells; after immunoprecipitation with different antibodies and others) in a study on the RAD51 paralogs' complexes and their role in genetic recombination (Masson et al., 2001).
Antibody first published in:
Masson et al. Identification and purification of two distinct complexes containing the five RAD51 paralogs. Genes Dev. 2001 Dec 15;15(24):3296-307. PMID:11751635
Note on publication:
This article describes the earliest use of XRCC2 7B7/3 (7B7/3) antibody to detect XRCC2 protein via Western blotting. However, the exact process of generation of this antibody is described in the following paper (where similar antibodies were generated): Masson, J. Y., Stasiak, A. Z., Stasiak, A., Benson, F. E., & West, S. C. (2001). Complex formation by the human RAD51C and XRCC3 recombination repair proteins. Proceedings of the National Academy of Sciences, 98(15), 8440-8446.
Immunofluorescence staining of HeLa cells using anti-XRCC2 antibody (Ab01306) 7B7/3. Immunofluorescence analysis of paraformaldehyde fixed HeLa cells permeabilized with 0.15% Triton and stained with the chimeric mouse IgG version of 7B7/3 (Ab01306-1.1) at 10 µg/ml followed by Alexa Fluor® 488 secondary antibody (2 µg/ml), showing nuclear staining. Actin filaments were stained with phalloidin (red) and the nuclear stain is DAPI (blue). Panels show from left-right, top-bottom Ab01306-1.1, DAPI, merged channels and an isotype control. The isotype control was stained with anti-unknown antibody (Ab00178-1.1) followed by Alexa Fluor® 488
Flow-cytometry using the anti-XRCC2 antibody 7B7/3 (Ab01306). HeLa cells were fixed using paraformaldehyde, permeabilised using 0.5% Triton and stained with anti-unknown IgG antibody (Ab00178-1.1; isotype control, black line) or the mouse IgG-chimeric version of 7B7/3 (Ab01306-1.1, blue line) at a dilution of 1:100 for 1h at RT. After washing, bound antibody was detected using a goat anti-mouse IgG AlexaFluor® 488 antibody at a dilution of 1:1000 and cells analyzed using a FACSCanto flow-cytometer.
Flow cytometry using the Anti-XRCC2 antibody 7B7/3 (Ab01306). Paraformaldehyde fixed HeLa cells permeabilized with 0.5% Triton were stained with anti-unknown specificity antibody (Ab00178-1.1; isotype control, black line) or the mouse IgG1 version of 7B7/3 (Ab01306-1.1, blue line) at a dilution of 1:100 for 1h at RT. After washing, the bound antibody was detected using a goat anti-mouse IgG AlexaFluor® 488 antibody at a dilution of 1:1000 and cells analyzed using a FACSCanto flow-cytometer.