United Kingdom (UK)
Recombinant monoclonal antibody to calf thymus ATPaseA. Manufactured using AbAb’s Recombinant Platform with variable regions (i.e. specificity) from the hybridoma 10 E8.
UniProt Accession Number of Target Protein: Q9TTA5
Alternative Name(s) of Target: SWI/SNF-related matrix-associated actin-dependent regulator of chromatin subfamily A-like protein 1; HepA-related protein; Sucrose nonfermenting protein 2-like 1
Immunogen: The mouse version of this antibody was generated by immunizing mice with immunopurified calf thymus ATPaseA.
Specificity: This antibody is specific for calf thymus ATPase A and recognizes the c-terminus of bovine and human homologs. Calf ATPaseA is a TP-dependent annealing helicase that binds selectively to fork DNA relative to ssDNA or dsDNA and catalyzes the rewinding of the stably unwound DNA.
Application Notes: The mouse version of this antibody was used for a western blot on calf DNA-dependent ATPase A isolated from calf thymus using immunoprecipitation (Mesner et al, 1993; pmid:8394114).
Antibody first published in:
Mesner et al. DNA-Dependent Adenosinetriphosphatase A: Immunoaffinity Purification and Characterization of Immunological Reagents-1 Biochemistry. 1993 Aug 3;32(30):7772-8. PMID:8394114
Note on publication:
An immunoaffinity purification of DNA-dependent ATPase A from fetal calf thymus is described.