United Kingdom (UK)
Recombinant monoclonal antibody to hINSR. Manufactured using AbAb’s Recombinant Platform with variable regions (i.e. specificity) from the hybridoma IR 18-146.
UniProt Accession Number of Target Protein: P06213
Alternative Name(s) of Target: CD220; human insulin receptor; insulin receptor; HHF5; IR; 18 146
Immunogen: This antibody was raised by immunising BALB/c mice with purified human placental insulin receptor.
Specificity: This confirmation-dependent antibody is specific for human INSR, reacting with an epitope within the CR domain of the alpha subunit (amino acids 191-290). It does not cross-react with rat, mouse or rabbit INSR, and displays low cross-reactivity with bovine INSR.
Application Notes: This antibody has been used in immunoprecipitation assays, with both radiolabelled human insulin receptor (hINSR) and hINSR-radiolabelled insulin complexes (Soos et al, 1986). This antibody has been shown to enhance insulin binding to IM-9 cells (Soos et al, 1986).
Antibody first published in:
Soos et al. Monoclonal antibodies reacting with multiple epitopes on the human insulin receptor. Biochem J. 1986 Apr 1; 235(1): 199–208. PMID:2427071
Note on publication:
Describes the original generation of this antibody, and the characterisation of its specificity.