United Kingdom (UK)
Recombinant monoclonal antibody to mCherry. Manufactured using AbAb’s Recombinant Platform with variable regions (i.e. specificity) from the llama peripheral lymphocytes LAM-2.
UniProt Accession Number of Target Protein: Q5S3G8
Alternative Name(s) of Target: mCh; RFP; Fluorescent protein raspberry; Red fluorescent protein; RFP-RANbody-2; LaM2
Immunogen: The original antibody was generated by immunization of Llama glama with mCherry fluorescent protein. A VHH cDNA library was generated by high-throughput DNA sequencing of a marrow lymphocyte from an immunized llama. Later on this was combined with mass spectrometric (MS) identification of high affinity VHH regions derived from serum of the same animal.
Specificity: This antibody specifically binds mCherry fluorescent protein.
Application Notes: This antibody is specific for mCherry and does not cross react with DsRed or TdTomato. This antibody can be used to detect mCherry fluorescent protein in an ELISA. This antibody binds immobilized mCherry with a binding affinity of Kd= 0.49 nM (PMID: 25362362).
Antibody first published in:
Fridy et al. A robust pipeline for rapid production of versatile nanobody repertoires. Nat Methods. 2014 Dec; 11(12): 1253–1260. PMID:25362362
Note on publication:
Describes the generation of large repertoires of readily expressible recombinant nanobodies with high affinities and specificities against a given antigen.