United Kingdom (UK)
Recombinant monoclonal antibody to nucleocapsid (N) protein. Manufactured using AbAb’s Recombinant Platform with variable regions (i.e. specificity) from the phage display antibody Nb1.
UniProt Accession Number of Target Protein: Q07499
Alternative Name(s) of Target: NC; N Protein; Nucleoprotein; Nucleocapsid protein;
Immunogen: The antibody was generated by immunizing a Bactrian camel with recombinant PEDV N protein followed by antibody library construction and phage display-based selection.
Specificity: The antibody binds the N protein of PEDV, which is associated with viral replication, transcription and assembly.
Application Notes: The specificity of this antibody (VHH) was confirmed by ELISA analysis. Langmiur model and sensorgrams data were used for kinetic analysis of PEDV N protein and Nb1. The Ka value measured was 1.97×10^5 M−1 s−1, indicating a fast association constant. The Ka/Kd value, the equilibrium dissociation constant between Nb1 and PEDV N protein, reached up to 0.96×10^8 M (Ma et al, 2019; pmid:31526383).
Antibody first published in:
Ma et al. A novel biotinylated nanobody-based blocking ELISA for the rapid and sensitive clinical detection of porcine epidemic diarrhea virus J Nanobiotechnology. 2019 Sep 16;17(1):96. PMID:31526383
Note on publication:
The paper describes the generation and characterization of the antibody.