United Kingdom (UK)
Recombinant monoclonal antibody to properdin. Manufactured using AbAb’s Recombinant Platform with variable regions (i.e. specificity) from the hybridoma 3A3E1.
UniProt Accession Number of Target Protein: P27918
Alternative Name(s) of Target: factor P; Complement factor P; properdin mab
Immunogen: The original antibody was generated by immunizing mice against purified human properdin.
Specificity: The antibody is specific for properdin. It recognizes human, baboon, rabbit, and canine properdin, but not mouse, rat, feline or ferret properdin.
Application Notes: The specificity of the original format of the antibody was confirmed by ELISA analysis. The antibody detected only non-reduced properdin as evaluated by western blot analysis. The antibody inhibited AP-mediated hemolysis of rabbit erythrocytes. The ability of the antibody to inhibit the binding of properdin to C3b was tested by flow cytometry. The antibody completely impaired the binding of properdin to C3b-coated erythrocytes (ESC3b) (Chen et al. 2020; PMID: 32793201). Further, the antibody inhibited PGA formation relative to TRAP alone (Blatt et al., 2016; PMID: 27183616).
Antibody first published in:
Chen et al. Properdin Is a Key Player in Lysis of Red Blood Cells and Complement Activation on Endothelial Cells in Hemolytic Anemias Caused by Complement Dysregulation Front Immunol. 2020 Jul 22;11:1460. PMID:32793201
Note on publication:
The original paper describes the generation and characterization of the antibody.