United Kingdom (UK)
Recombinant monoclonal antibody to virus particles. Manufactured using AbAb’s Recombinant Platform with variable regions (i.e. specificity) from the phage display antibody A1.
Alternative Name(s) of Target: WSSP
Immunogen: The original antibody was generated by immunizing mice with WSSV followed by antibody library construction and phage display-based selection.
Specificity: The antibody is specific for WSSV. The antibody binds to native WSSV, and not to the denatured WSSV.
Application Notes: The binding specificity of the scFv antibody was determined by ELISA (Dai et al., 2003; PMID: 12969566 and Yuan et al., 2006; PMID: 17140131). The antibody had an affinity constant (Kaff) of 2.02 x 10^9 M-1. Dot blot assays showed that the antibody could detect WSSV directly from shrimp hemolymph after 24-h feeding infection by WSSV (Dai et al., 2003; PMID: 12969566).
Antibody first published in:
Dai et al. Construction and characterization of a novel recombinant single-chain variable fragment antibody against White Spot Syndrome Virus from shrimp J Immunol Methods. 2003 Aug;279(1-2):267-75. PMID:12969566
Note on publication:
The original paper describes the generation and characterization of the antibody by phage display method.