United Kingdom (UK)
Recombinant monoclonal antibody to alpha-Gal. Manufactured using AbAb’s Recombinant Platform with variable regions (i.e. specificity) from the hybridoma Gal13.
Alternative Name(s) of Target: alpha gal; Galα1–3Galβ1–4GlcNAc-R; alpha gal; gal; α-gal
Immunogen: This antibody was raised by immunising BALB/C mice, which express α-gal epitopes, with rabbit red cell membranes, on which α-gal is the major carbohydrate epitope.
Specificity: This antibody is specific for α-gal epitopes (Galα1–3Galβ1–4GlcNAc-R) on glycosphingolipids.
Application Notes: This antibody binds to synthetic α-gal epitopes linked to BSA and to α-gal epitopes on glycolipid molecules from rabbit and bovine erythrocytes (Chen et al, 2000; Galili et al, 1987). However, in contrast to monoclonal anti-Gal antibodies originating from α1,3GT knockout mice [M86; B1H2; B4H2], this antibody does not bind to α-gal epitopes on carbohydrate chains of natural glycoproteins. This antibody has been used to confirm that Galαl-3Gal glycosphingolipids are expressed at low levels by human erythrocytes (Galili et al, 1987).
Antibody first published in:
Galili et al. Identification of erythrocyte Gal alpha 1-3Gal glycosphingolipids with a mouse monoclonal antibody, Gal-13. J Biol Chem. 1987 Apr 5;262(10):4683-8. PMID:2435715
Note on publication:
Describes the original generation of this antibody, and its characterisation in ELISA analysis.