United Kingdom (UK)
Recombinant monoclonal antibody to (1-4)-β-D-galactan. Manufactured using AbAb’s Recombinant Platform with variable regions (i.e. specificity) from the hybridoma LM5.
Immunogen: The rat version of this antibody was raised by immunizing a wister rat with a polysaccharide.
Specificity: This antibody is specific for beta-1,4-galactan. It specifically recognized more than 3 contiguous units of beta-1,4-galactan. Beta-1,4-galactan is expressed prefominantly in the cell wall.
Application Notes: To characterize this antibody, a competitive ELISA was performed on saccharides using the original rat version of this antibody. Furthermore, an immunoblot was performed on citrus pectin, apple pectin, TG1, TG2a, TG2b, GALCBSA, BSA, lupin galactan, gum arabic, polygalacturonic acid, larchwood arabinogalactan, and locust bean gum using this antibody. Finally, tomato pericarp was immunogold labelled using this antibody (Jones et al, 1997; pmid:12223681). To map the epitope of this antibody, an ELISA was performed on different lengths of beta-1,4-galactan and beta-1,4-galactan with different side groups. It showed the greatest binding efficiency to 4 units of beta-1,4-galactan (Andersen et al, 2016; pmid:27855335). While creating and characterizing an antibody against (1→5)-α-l-arabinan, the rat version of this antibody was used as a control in an ELISA. Furthermore, a immunoblot was performed on surfaces of lemons using this antibody (Willats et al, 1998, pmid:9675359). Pea cotyledons were stained for immunofluorescence using the rat version of this antibody to study plant cell wall architecture. Furthermore, immunogold assay was performed on pea cotyledons using this antibody (McCartney et al, 2000; pmid:10792826).
Antibody first published in:
Jones et al. Localization of Pectic Galactan in Tomato Cell Walls Using a Monoclonal Antibody Specific to (1[->]4)-[beta]-D-Galactan Plant Physiol. 1997 Apr;113(4):1405-1412. PMID:12223681
Note on publication:
Antibodies againt saccharides were created and characterized.