United Kingdom (UK)
Recombinant monoclonal antibody to 14G2a. Manufactured using AbAb’s Recombinant Platform with variable regions (i.e. specificity) from the hybridoma 4B5.
Alternative Name(s) of Target: ganglioside; ganglioside 2a
Immunogen: The original human version of this antibody was generated by treating humans with the 14G2a antibody then MNCs were harvested and hybridized with the murine myeloma cell line P3X.Ag8.653.
Specificity: This antibody is specific for 14G2A. 14G2A is a mouse IgG2a antibody specific for human GD2.
Application Notes: To determine the functionality of the mouse version of this antibody an ELISA was preformed to test if this antibody was able to bind to antigen 14G2A. Futhermore the antibody was also tested in a western blot on antigen 14G2A (EP1000082A2). To screen for anti-14G2a activity of the human version of this antibody a radioimmunoassay was preformed (Saleh et al, 1993; pmid:8376782). To asses the ability of the human version of this antibody to mediate a cellular immune response. Rabbits were immunized with 4B5. Then irradiated Mel-21 cells were inplanted. The result was induration at the site of the irradiated cells (Saleh et al, 1993; pmid:8376782).
Antibody first published in:
Saleh et al. Generation of a human anti-idiotypic antibody that mimics the GD2 antigen J Immunol. 1993 Sep 15;151(6):3390-8. PMID:8376782
Note on publication:
Four human anti-14G2a secreting hybridomas were generated and the mAb product of one of the hybridomas was characterized.