United Kingdom (UK)
Recombinant monoclonal antibody to 3-pHis. Manufactured using AbAb’s Recombinant Platform with variable regions (i.e. specificity) from the hybridoma SC44-1.
Alternative Name(s) of Target: 3-phosphohistidine; N3-Phosphohistidine; histidine
Immunogen: The original rabbit version of this antibody was raised by immunizing rabbits with a 3-pTza library conjugated to the carrier protein keyhole limpet hemocyanin (KLH).
Specificity: This antibody is specific for 3-pHis and does not cross react with phosphotyrosine (pTyr) or the other pHis isomers.
Application Notes: This antibody can be used for the identification of 3-pHis substrates and functional study of pHis. It was shown to detect various proteins containing 3-pHis, such as SCS and ACLY (Fuhs et al, 2015; pmid: 26140597). SC44-1 was also used for phosphoenrichment using immunoaffinity purification (Hunter et al., 2019). This clone was used to analyse the prevalence of 3-pHis in hepatocellular carcinoma by Western blot (Hindupur et al., 2018; pmid: 29562234). In the same study, SC44-1 was further utilized to demonstrate that LHPP is a protein histidine phosphatase (Hindupur et al., 2018; pmid: 29562234).
Antibody first published in:
Fuhs et al. Monoclonal 1- and 3-Phosphohistidine Antibodies: New Tools to Study Histidine Phosphorylation Cell. 2015 Jul 2; 162(1): 198–210. PMID:26140597
Note on publication:
Describes the generation and characterization of the monoclonal antibodies (mAbs) that specifically recognize 1-pHis or 3-pHis.