Anti-ACTB/NUCL complex [Nb10]

Description:

Recombinant monoclonal antibody to ACTB/NUCL complex. Manufactured using AbAb’s Recombinant Platform with variable regions (i.e. specificity) from the alpaca peripheral blood lymphocytes Nb10.

Clone Name:

Nb10

Published Application(s):

WB, ELISA

Published Species Reactivity:

Human

UniProt Accession Number of Target Protein: P60709; P19338

Alternative Name(s) of Target: ACTB; Actin; Beta-actin; β-actin; NCL; Nucleolin; Protein C23
Immunogen: The original antibody was isolated from a VHH library generated by immunization of an alpaca with whole glioblastoma multiforme (GBM) cells.

Specificity: This antibody binds the β-actin/nucleolin (ACTB/NUCL) complex. Actin is a highly conserved protein that polymerizes to produce filaments that form cross-linked networks in the cytoplasm of cells. Nucleolin is a major nucleolar protein of growing eukaryotic cells. It is found associated with intranucleolar chromatin and pre-ribosomal particles. It is thought to play a role in pre-rRNA transcription and ribosome assembly.

Application Notes: This antibody was used for identification of ACTB/NUCL complex in cytosolic and membrane protein fractions of glioblastoma (GBM) tissues using western blotting. It was reported that NUCL and ACTB were poorly expressed in cytosolic GBM protein fractions and showed increased expression in GBM membrane protein fractions. ELISA was used for the screening of the nanobody library and identification of specific binders that showed specificity for GBM proteins (PMID: 28498803).

Antibody first published in: Jovčevska et al. Differentially expressed proteins in glioblastoma multiforme identified with a nanobody-based anti-proteome approach and confirmed by OncoFinder as possible tumor-class predictive biomarker candidates. Oncotarget. 2017 Jul 4;8(27):44141-44158. PMID:28498803
Note on publication: Describes the isolation of nanobodies against various antigens overexpressed in glioblastoma multiforme.

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