United Kingdom (UK)
Recombinant monoclonal antibody to AIF1. Manufactured using AbAb’s Recombinant Platform with variable regions (i.e. specificity) from the hybridoma GT1-mAb2.
UniProt Accession Number of Target Protein: P55008
Alternative Name(s) of Target: AIF1; IBA1; allograft inflammatory factor 1; AIF-1; IRT-1; interferon gamma responsive transcript; ionized calcium-binding adapter molecule 1; Daintain; DADB-70P7.8; allograft inflammatory factor-1 splice variant Hara-1; IRT1; protein G1
Immunogen: This antibody was obtained by sequencing a polyclonal goat IgG raised against a peptide with the sequence C-TGPPAKKAISELP.
Specificity: This antibody is specific to EBP05419, an immunizing peptide with the sequence C-TGPPAKKAISELP. This peptide was used in the production of the polyclonal Goat Anti-AIF1 antibody from which this antibody is derived.
Application Notes: This antibody (GT1-mAb2) was one of two monoclonal antibodies discovered by analyzing a polyclonal antibody (GT-1) sample. Briefly, de novo sequencing of GT-1 resulted in the full-length sequences of two distinct monoclonal antibodies (GT1-mAb1 and GT1-mAb2) in both framework and CDR regions. Both antibodies were recombinantly expressed by Absolute Antibody in HEK293 cells and tested in various assays. GT1-mAb2 was tested against the peptide antigen in an ELISA, and it showed activity comparable to that of the original polyclonal antibody. GT1-mAb2 was found to bind the target peptide in a non-competitive manner with GT1-mAb1 while being efficiently blocked by the original polyclonal antibody, proving that both monoclonal antibodies bind distinct epitopes and that GT1-mAb2 possess binding characteristics of the input polyclonal antibody. Furthermore, GT1-mAb2 was found to be compatible with immunofluorescence (IF) and flow cytometry (FC), although with an FC signal observedly less distinct than that of GT1-mAb1.
Antibody first published in: PMID: