Anti-Alpha actin 1 [3G8C7A6]

Name: Anti-Alpha actin 1 [3G8C7A6]
SKU: Ab03147
Availability: InStock

Description:

Recombinant monoclonal antibody to Alpha actin 1. Manufactured using AbAb’s Recombinant Platform with variable regions (i.e. specificity) from the hybridoma 3G8C7A6.

Clone Name:

3G8C7A6

Published Application(s):

IHC-P, ELISA

Published Species Reactivity:

Human

Products based on this clone:

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Ab03147-23.0 Anti-Alpha actin 1 [3G8C7A6]
Human
Rabbit IgG
Purified
In Stock

Ab03147-10.0 Anti-Alpha actin 1 [3G8C7A6]
Human
Human IgG1
Purified
Ships in 4-5 weeks

Ab03147-10.3 Anti-Alpha actin 1 [3G8C7A6]
Human
Human IgG1
Fc Silent™
Purified
Ships in 4-5 weeks

Ab03147-3.0 Anti-Alpha actin 1 [3G8C7A6]
Human
Mouse IgG2b
Purified
In Stock

Ab03147-3.3 Anti-Alpha actin 1 [3G8C7A6]
Human
Mouse IgG2b
Fc Silent™
Purified
Ships in 4-5 weeks

UniProt Accession Number of Target Protein: P68133

Alternative Name(s) of Target: Actin; ACTA1; ACTA; Actin alpha skeletal muscle; Alpha-actin-1; Muscle specific actin; MSA; HHF35; CGMCNO.22312; HHF35
Immunogen: The original antibody was generated by immunizing BALB/c mice with a recombinant human actin protein.

Specificity: This antibody binds actin. Actin is a type of spherical multi-functional protein that forms a microfilament. Actin is widely existent in eukaryotic organisms, and there is a major component constituting the muscle segment and the cell skeleton in the cytoplasm and cellular nucleus of the intramuscular cell. Muscle specific actin (MSA) is a highly conserved, ubiquitous protein found in muscle and some non-muscle cells. In addition to participating in the normal morphology of maintaining cells, actin also participates in the formation, adhesion, migration, apoptosis, material transport, transmembrane information transfer, and receptor aggregation.

Application Notes: This antibody is highly similar to the commercially available anti-actin antibody HHF35. This antibody can use used in the immunohistochemical staining of smooth fibroids. This antibody can be used in the identification of actin from various tissues including brain, heart, cerebellum, esophageal, adrenal, stomach, ovaries, small intestines, pancreatic, colorectal, parathyroid, liver, pituitary, salivary gland, testicular, kidney, thyroid, prostate, breast, uterus, spleen, bladder, tonsil, skeletal muscle, thymus (young children), skin, bone marrow, peripheral nerve, lung, melodder cells. The binding specificity of this antibody towards actin was determined using ELISA. The original IgG2b antibody binds actin with an affinity constant of 6.26X10^9 M^(-1) (CN113817055).

Antibody first published in: PMID:

Flow cytometry analysis of HeLa cells using Ab03147-23.0. Click on image for more details.

Flow cytometry using anti-Alpha actin 1 antibody 3G8C7A6 (Ab03147). Paraformaldehyde fixed HeLa cells permeabilized with 0.5% Triton were stained with the anti-unknown specificity antibody (Ab00178-23.0; isotype control, black line) or the rabbit IgG version of 3G8C7A6 (Ab03147-23.0, blue line) at a dilution of 1:100 for 1h at RT. After washing, the bound antibody was detected using a goat anti-rabbit IgG AlexaFluor® 488 antibody at a dilution of 1:1000 and the cells were analyzed using a FACSCanto flow-cytometer.

Western blot of HEK293 and HeLa cells lysate and human skeletal muscle tissue lysate using Ab03147-23.0. Click on image for more details.

Western blot using anti-Alpha actin 1 antibody 3G8C7A6 (Ab03147). HEK293 (A) (1µg/ml), HeLa (B) (0.1µg/ml) cells lysate, and human skeletal muscle (C) (0.01µg/ml) tissue lysate (35µg protein in RIPA buffer) were resolved on an SDS-PAGE gel, and blots were probed with the chimeric rabbit version of 3G8C7A6 (Ab03147-23.0) at the mentioned respective concentrations before detection using an anti-rabbit secondary antibody. A primary incubation of 1 hour was used, and protein was detected by chemiluminescence.

Immunofluorescence staining of HeLa cells using Ab03147-23.0. Click on image for more details.

Immunofluorescence staining of HeLa cells with anti-Alpha actin 1 antibody 3G8C7A6 (Ab03147). Immunofluorescence analysis of paraformaldehyde fixed HeLa cells, permeabilized with 0.15% Triton, on Shi-fix™ coverslips stained with the chimeric rabbit IgG version of 3G8C7A6 (Ab03147-23.0) (1:100 dilution) for 1h followed by Alexa Fluor® 488 secondary antibody (1:1000 dilution), showing cytoplasmic staining. The nuclear stain is DAPI (blue). Panels show, from left-right, top-bottom, Ab03147-23.0, DAPI, merged channels, and an isotype control. The isotype control was an unknown specificity antibody (Ab00178-23.0) followed by staining with Alexa Fluor® 488 secondary antibody.

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