United Kingdom (UK)
Recombinant monoclonal antibody to Alpha-crystallin. Manufactured using AbAb’s Recombinant Platform with variable regions (i.e. specificity) from the phage display antibody B4m3.
UniProt Accession Number of Target Protein: P9WMK1
Alternative Name(s) of Target: Acr; hspX; 14 kDa antigen; 16 kDa antigen; Antigen 16kDa (HspX); HSP 16.3; Nox16
Immunogen: This antibody was generated by first panning the Nkuku® library [26] against 20 μg/ml of the M. tuberculosis 16 kDa antigen. Second a sublibrary was created using error-prone PCR. Finally the mutant library was panned against 16 kDa M. tuberculosis antigen.
Specificity: This antibody is specific for the HspX antigen from Mycobacterium tuberculosis. This antigen acts as a chaperone, as it has a significant ability to suppress the thermal denaturation of alcohol dehydrogenase. Mycobacterium tuberculosis causes the disease tuberculosis.
Application Notes: The binding of the scFv version of this antibody to the antigen was characterised by ELISA. Furthermore, surface plasmon resonance was used to determine the binding kinetics of this antibody on 16 kDa protein of M. tuberculosis (Sixholo et al, 2011; pmid:21349739).
Antibody first published in:
Sixholo et al. Improving the characteristics of a mycobacterial 16 kDa-specific chicken scFv. Biologicals. 2011 Mar;39(2):110-6. PMID:21349739
Note on publication:
A chicken single chain variable fragment (scFv) phage display library was panned against the mycobacterial 16 kDa antigen. Three fusion phages which bound specifically to the antigen were selected, each of which produced low signals in ELISA when secreted as a soluble scFv. One scFv was therefore chosen to be modified in an attempt to improve its binding.