Anti-Arginase 1 [mAb5]

Name: Anti-Arginase 1 [mAb5]
SKU: Ab03081
Availability: InStock

Description:

Recombinant monoclonal antibody to Arginase 1. Manufactured using AbAb’s Recombinant Platform with variable regions (i.e. specificity) from the hybridoma mAb5.

Clone Name:

mAb5

Published Application(s):

Cryo-electron microscopy, inhibition

Published Species Reactivity:

Human

Products based on this clone:

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Ab03081-23.0 Anti-Arginase 1 [mAb5]
Human
Rabbit IgG
Purified
In Stock

Ab03081-1.1 Anti-Arginase 1 [mAb5]
Human
Mouse IgG1
Purified
Ships in 5-6 weeks

UniProt Accession Number of Target Protein: P05089

Alternative Name(s) of Target: ARG1; Arginase-1; Liver-type arginase; Type I arginase
Immunogen: The antibody was generated by immunizing mice with recombinant arginase 1.

Specificity: The antibody binds arginase 1. The binding of the antibody does not sterically block the Arginase 1 active site, but it interacts with an amino acid (Arg21) important to substrate binding by arginase 1, thereby inhibiting enzyme activity. Arginase is a manganese-containing enzyme that catalyzes the hydrolysis of L-arginine to L-ornithine and urea, and modulates T-cell-mediated immune response.

Application Notes: The structure and epitope mapping of the complex arginase:antibody was determined by cryo-electron microscopy. The antibody was assessed for its potency and extent of inhibition of mouse arginase using an assay that detects the formation of thioornithine from thioarginine. In this assay, as well as an LCMS assay, it was determined that the antibody could fully inhibit the enzymatic activity of arginase. To assess the kinetic mechanism of inhibition of the antibody, increasing fixed concentrations of antibody were tested across a range of thioarginine concentrations. The Ki of the antibody against mouse Arg1 was 25 ± 1.7 nM (Palte et al., 2021; PMID: 34326456).

Antibody first published in: Palte et al. Cryo-EM structures of inhibitory antibodies complexed with arginase 1 provide insight into mechanism of action. Commun Biol. 2021 Jul 29;4(1):927. PMID:34326456
Note on publication: The paper describes the generation and characterization of the antibody. The antibody was used as probes for Human Arginase 1 conformational study.

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