United Kingdom (UK)
Recombinant monoclonal antibody to Ataxin-1, 11NQ. Manufactured using AbAb’s Recombinant Platform with variable regions (i.e. specificity) from the hybridoma N76/8.
UniProt Accession Number of Target Protein: P54254
Alternative Name(s) of Target: N76/8R; Atxn1; Spinocerebellar ataxia type 1 protein homolog; Ataxin-1
Immunogen: This antibody was raised by immunising BALB/c mice with a synthetic peptide amino acids 164-197 of mouse ataxin-1.
Specificity: This antibody is specific for amino acids 164-197 of HCN1. It shows no cross-reactivity against HCN2. HCN1 is a chromatin-binding factor that repress Notch signaling in the absence of Notch intracellular domain by acting as a CBF1 corepressor.
Application Notes: This antibody has been used in a western blot experiment on mouse brain lysates and HeLa cell lysates (Suh et al, 2019; PMID:31442405) (Barclay et al, 2014; PMID:24179173). Immunohistochemistry was preformed with this antibody on the brain of mice (Suh et al, 2019; PMID:31442405). The antibody has shown use immunoprecipitation experiments of HeLa cell lysate (Barclay et al, 2014; PMID:24179173).
Antibody first published in:
Barclay et al. Systems biology analysis of Drosophila in vivo screen data elucidates core networks for DNA damage repair in SCA1 Hum Mol Genet. 2014 Mar 1;23(5):1345-64. PMID:24179173
Note on publication:
they performed a systematic in vivo screen of all available Drosophila melanogaster homolog DNA repair genes, and we tested the effect of their overexpression on lifespan and developmental viability in Spinocerebellar Ataxia Type 1 (SCA1) Drosophila models expressing human mutant Ataxin-1 (Atxn1).