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- Ab03193-23.0 Anti-B-catenin [3B1D11A8]
- Human
- Rabbit IgG
- Purified
- In Stock
- Ab03193-1.1 Anti-B-catenin [3B1D11A8]
- Human
- Mouse IgG1
- Purified
- In Stock
- Ab03193-10.0 Anti-B-catenin [3B1D11A8]
- Human
- Human IgG1
- Purified
- Ships in 4-5 weeks
Recombinant monoclonal antibody to B-catenin. Manufactured using AbAb’s Recombinant Platform with variable regions (i.e. specificity) from the hybridoma 3B1D11A8.
UniProt Accession Number of Target Protein: P35222
Alternative Name(s) of Target: CTNNB1; CTNNB; Catenin beta-1; Beta-catenin; CAT-5H10; CGMCC No. 19685
Immunogen: The original antibody was generated by immunizing BALB/c mice with human beta-catenin-KLH conjugate in Freund's non-complete adjuvant.
Specificity: This antibody binds human beta-catenin. The human β-catenin acts as a multi-functional protein that plays a very important role in cell adhesion and Wnt signaling pathway mediated gene expression regulation. Β-catenin on the cell membrane can be combined with the cytoplasmic domain of E-Cadherin (E-CAD) to form an e-cadherin / β-catenin complex, which mediates mutual adhesion between the same type. It acts as a key regulatory molecule in the Wnt signaling pathway and plays an important role in the occurrence / evolution of the embryonic development and tumor.
Application Notes: The binding characterization of this antibody was done using ELISA. The original mouse IgG1 antibody binds human B-catenin with an affinity constant of 4.96 X 10^(9) M^(-1). This antibody was also used in the immunohistochemical analysis of colorectal carcinoma and pancreatic cancer tissues. The immunohistochemical staining pattern of this antibody is similar to commercially available antibody CAT-5H10 (CN112457400).
Antibody first published in: PMID:
Immunofluorescence staining of HeLa cells with anti-B-catenin antibody 3B1D11A8 (Ab03193). Immunofluorescence analysis of paraformaldehyde fixed HeLa cells on Shi-fix™ coverslips stained with the chimeric rabbit IgG version of 3B1D11A8 (Ab03193-23.0) (1:100 dilution) for 1 hour followed by Alexa Fluor® 488 secondary antibody (1:1000 dilution), showing cytoplasmic and membrane staining. The nuclear stain is DAPI (blue). Panels show, from left to right, top to bottom, Ab03193-23.0, DAPI, merged channels, and an isotype control. The isotype control was an unknown specificity antibody (Ab00178-23.0) followed by staining with Alexa Fluor® 488 secondary antibody.
Western blot using anti-B-catenin antibody 3B1D11A8 (Ab03193). MCF7 (A) and Caco-2 (B) cells lysates, human skin (C), and human breast cancer (D) tissue lysates (35µg protein in RIPA buffer) were resolved via SDS-PAGE, and the subsequent blots were probed with the chimeric rabbit version of 3B1D11A8 (Ab03193-23.0) at 0.01µg/ml before detection using an anti-rabbit secondary antibody. A primary incubation of 1 hour was used, and proteins were detected by chemiluminescence.