- Ab03962-23.0 Anti-BAX [3C10]
- Rabbit IgG
- Ships in 5-6 weeks
Recombinant monoclonal antibody to BAX. Manufactured using AbAb’s Recombinant Platform with variable regions (i.e. specificity) from the hybridoma 3C10.
UniProt Accession Number of Target Protein: Q07812
Alternative Name(s) of Target: Apoptosis regulator BAX; Bcl-2-like protein 4 (Bcl2-L-4)
Specificity: The antibody is specific for Bax. It recognizes a peptide encompassing residues 31–45 at the start of Bax a1–a2 loop (AAs RAGRM).
Application Notes: The specificity of the antibody for Bax was determined by ELISA analysis. The antibody was able to bind to mitochondrial Bax and WT Bax by western blot analysis. The antibody immunoprecipitated Bax (Iyer et al., 2016; PMID: 27217060). The crystal structure of the Fab fragment in complex with BAX was determined. The antibody activates mitochondrial Bax, but blocks translocation of cytosolic Bax (Robin et al., 2018; PMID: 30122452).
Antibody first published in:
Robin et al. Identification of an activation site in Bak and mitochondrial Bax triggered by antibodies. Nat Commun. 2016 May 24;7:11734. doi: 10.1038/ncomms11734. PMID:2721706
Note on publication: The original paper describes a panel of antibodies that activates Bak and mitochondrial Bax.