United Kingdom (UK)
Recombinant monoclonal antibody to Benzopyrene. Manufactured using AbAb’s Recombinant Platform with variable regions (i.e. specificity) from the hybridoma 22F12.
Alternative Name(s) of Target: B[a]P; PAHs; PAH; Benzo[a]pyrene; 3 4-Benzopyrene; polycyclic aromatic hydrocarbons; chrysene; indeno[1 2 3-cd]pyrene; benzo[b]fluoranthene
Immunogen: The original antibody was generated by using six B[a]P-protein conjugates B[a]P-6-C4-BSA, B[a]P-6-C4-KLH, B[a]P-7-C4-BSA, B[a]P-7-C4-KLH, B[a]P-10-C4-BSA and B[a]P-10-C4-KLH as immunogens for mouse immunizations.
Specificity: This antibody is specific for benzo[a]pyrene, which is an organic compound belonging to polycyclic aromatic hydrocarbon (PAH). Benzo[a]pyrene is one of the isomeric forms of benzopyrene. It comes from certain substances when they are not burned completely. It is found in car exhaust, smoke from wood fires, tobacco, oil and gas products, charred or grilled foods, and other sources. Traces of Benzopyrene are found in soil and water. It is also a causative agent for skin rashes, burning feeling, skin color changes, warts, bronchitis and even lung cancers.
Application Notes: This antibody was used in the development of a sensitive ELISA for the detection of benzopyrene in water. The average IC50 for B[a]P and corresponding detection limit at a signal : noise (S/N) ratio of 3 was 65 ng/L and 24 ng/L. This antibody also cross reacts with chrysene,indeno[1,2,3-cd]pyrene, and benzo[b]fluoranthene (PMID: 15965533).
Antibody first published in:
Matschulat et al. Development of a highly sensitive monoclonal antibody based ELISA for detection of benzo[a]pyrene in potable water. Analyst. 2005 Jul;130(7):1078-86. PMID:15965533
Note on publication:
This paper describes the development of a sensitive indirect competitive ELISA for the detection of B[a]P in potable water.