United Kingdom (UK)
Recombinant monoclonal antibody to Biotin. Manufactured using AbAb’s Recombinant Platform with variable regions (i.e. specificity) from the hybridoma BK-1/39.
Alternative Name(s) of Target: vitamin B7; vitamin H; coenzyme R
Immunogen: This antibody was raised by immunising mice with biotin conjugated to keyhole limpet haemocyanin.
Specificity: This antibody recognises biotin (vitamin B7). It is a water-soluble vitamin which serves as a cofactor of important metabolic enzymes involved in processing of carbohydrates, lipids and amino acids as well as carbon dioxide transport.
Application Notes: This monoclonal antibody can be used to detect biotinylated proteins and antibodies, particularly if signal amplification is desired. For instance, PE-conjugated BK-1/39 antibody was used to target biotinylated anti-Ig beta antibody in a flow cytometric analysis (Levit-Zerdoun et al., 2016). This antibody was also successfully utilised in the chromatin immunoprecipitation (ChIP) of biotinylated sense or antisense strands of the saRNA duplex (Wu et al., 2016). BK-1/39 was used in in situ hybridisation as well; it detected biotinylated RNA probe complementary to the Epstein-Barr virus genome (de Lima et al., 2012).
Antibody first published in:
Arnholdt et al. Receptor-mediated processing of epidermal growth factor in the trophoblast of the human placenta. Virchows Arch B Cell Pathol Incl Mol Pathol. 1991;61(2):75-80. PMID:1683723
Note on publication:
This article describes the earliest found application of BK-1/39 antibody.
ELISA using BK-1/39 (Ab01346) and biotin. Binding curves of the rabbit IgG chimeric version of the Anti-Biotin antibody BK-1/39 (Ab01346-1.1; green line) and isotype control (Ab00102-23.0, anti-fluorescein; orange line) to an ELISA plate coated with biotin at a concentration of 2.5 ug/ml. A 3-fold serial dilution from 3000 to 0.0169 ng/ml was performed using Ab01346 antibody. For signal detection, a 1:4000 dilution of a HRP-conjugated anti-rabbit IgG antibody was used.