- Ab00748-1.1 Anti-BSA [D1]
- Mouse IgG1
- In Stock
- Ab00748-23.0 Anti-BSA [D1]
- Rabbit IgG
- In Stock
Recombinant monoclonal antibody to BSA. Manufactured using AbAb’s Recombinant Platform with variable regions (i.e. specificity) from the phage display antibody D1.
UniProt Accession Number of Target Protein: P02769
Alternative Name(s) of Target: Bovine serum albumin; serum albumin; albumin
Immunogen: D1 was prepared by isolating anti-p24 scFvs from a phage display library made from a mouse immunized with lysate from HIV-1 infected H9 cells.
Specificity: D1 has been shown to bind to BSA in panning experiments from phage display. Albumin is a secreted globular protein found in blood plasma and BSA has multiple biochemical applications including ELISAs and immunohistochemistry and is able to increase the stability of proteins and be used as a standard in Bradford assays.
Application Notes: D1 was selected for by panning where it reacted postiively on BSA. D1 can also be used as a control in IP and when working with native proteins when conducting studies within a BSA medium.
Antibody first published in:
de Haard et al. Selection of recombinant, library-derived antibody fragments against p24 for human immunodeficiency virus type 1 diagnostics. Clin Diagn Lab Immunol. 1998 Sep;5(5):636-44. PMID:9729530
Note on publication: Describes the production of the anti-BSA antibody D1 in addition to antibodies specific for the viral protein p24.
ELISA of anti-BSA antibody on BSA. Binding curves of the rabbit IgG chimeric version of the anti-BSA antibody D1 (Ab00748-23.0; blue line) and isotype control (Ab00102-23.0, anti-fluorescein; orange line) to an ELISA plate coated with BSA at a concentration of 5 µg/ml. A 3-fold serial dilution from 90 µg/ml was tested in the experiment. For signal detection, a 1:4000 dilution of an HRP-conjugated anti-rabbit IgG antibody was used.