United Kingdom (UK)
Recombinant monoclonal antibody to CA125. Manufactured using AbAb’s Recombinant Platform with variable regions (i.e. specificity) from the hybridoma 20E3.
UniProt Accession Number of Target Protein: Q8WXI7
Alternative Name(s) of Target: TA347; Mucin-16; MUC-16; Ovarian cancer-related tumor marker CA125; CA-125; Ovarian carcinoma antigen CA125; CGMCC NO 20773
Immunogen: The original antibody was generated by immunizing mice with recombinant protein CA125.
Specificity: The original antibody is specific for CA125. CA125 is thought to provide a protective, lubricating barrier against particles and infectious agents at mucosal surfaces. This antigen that is the basis for a widely used serum assay for the monitoring of patients with ovarian epithelial cancer.
Application Notes: The antibody 20E3 and the commercial antibody TA347 were used to stain ovarian slurry adenocarcinoma and ovarian mucous adenocarcinoma in immunohistochemistry. Results showed the antibody specificity is comparable to the commercial antibody. Further, the staining intensity of the antibody 20E3 was higher than the commercial antibody (CN113061186A).
Antibody first published in: PMID:
Immunofluorescence staining of HeLa cells with anti-CA125 antibody 20E3 (Ab03309). Immunofluorescence analysis of paraformaldehyde fixed HeLa cells on Shi-fix™ coverslips stained with the chimeric rabbit IgG version of 20E3 (Ab03309-23.0) (1:100 dilution) for 1h followed by Alexa Fluor® 488 secondary antibody (1:1000 dilution), showing membrane staining. The nuclear stain is DAPI (blue). Panels show, from left-right, top-bottom, Ab03309-23.0, DAPI, merged channels, and an isotype control. The isotype control was an unknown specificity antibody (Ab00178-23.0) followed by staining with Alexa Fluor® 488 secondary antibody.
Flow cytometry using anti-CA125 antibody 20E3 (Ab03309). HeLa cells were stained with the anti-unknown specificity antibody (Ab00178-23.0; isotype control, black line) or the rabbit IgG version of 20E3 (Ab03309-23.0, blue line) at a dilution of 1:100 for 1h at RT. After washing, the bound antibody was detected using a goat anti-rabbit IgG AlexaFluor® 488 antibody at a dilution of 1:1000, and the cells were analyzed using a FACSCanto flow-cytometer.