United Kingdom (UK)
Recombinant monoclonal antibody to calf thymus ATPaseA. Manufactured using AbAb’s Recombinant Platform with variable regions (i.e. specificity) from the hybridoma 3B2-A2-E5.
UniProt Accession Number of Target Protein: Q9TTA5
Alternative Name(s) of Target: SWI/SNF-related matrix-associated actin-dependent regulator of chromatin subfamily A-like protein 1; HepA-related protein; Sucrose nonfermenting protein 2-like 1
Immunogen: The mouse version of this antibody was generated by immunizing mice with immunopurified calf thymus ATPaseA.
Specificity: This antibody is specific for calf thymus ATPase A and recognizes proteins containing both a polynucleotide binding site and a nucleotide binding site. Calf ATPaseA is a TP-dependent annealing helicase that binds selectively to fork DNA relative to ssDNA or dsDNA and catalyzes the rewinding of the stably unwound DNA.
Application Notes: The mouse version of this antibody was used for a western blot on calf DNA-dependent ATPase A isolated from calf thymus using immunoprecipitation (Mesner et al, 1993; pmid:8394114).
Antibody first published in:
Mesner et al. DNA-Dependent Adenosinetriphosphatase A: Immunoaffinity Purification and Characterization of Immunological Reagents-1 Biochemistry. 1993 Aug 3;32(30):7772-8. PMID:8394114
Note on publication:
An immunoaffinity purification of DNA-dependent ATPase A from fetal calf thymus is described.