United Kingdom (UK)
Recombinant monoclonal antibody to calf thymus ATPaseA. Manufactured using AbAb’s Recombinant Platform with variable regions (i.e. specificity) from the hybridoma 4B10-A3-E4.
UniProt Accession Number of Target Protein: Q9TTA5
Alternative Name(s) of Target: SWI/SNF-related matrix-associated actin-dependent regulator of chromatin subfamily A-like protein 1; HepA-related protein; Sucrose nonfermenting protein 2-like 1
Immunogen: The mouse version of this antibody was generated by immunizing mice with immunopurified calf thymus ATPaseA.
Specificity: This antibody is specific for calf thymus ATPase A and recognizes bovine, human, and mouse homologs. Calf ATPaseA is a TP-dependent annealing helicase that binds selectively to fork DNA relative to ssDNA or dsDNA and catalyzes the rewinding of the stably unwound DNA. The antibody also shows a reaction to the denatured protein.
Application Notes: The mouse version of this antibody was used for a western blot on calf DNA-dependent ATPase A isolated from calf thymus using immunoprecipitation (Mesner et al, 1993; pmid:8394114). To identify the mouse version of this antibody an ELISA on native ATPase A was preformed. Furthermore, a western blot was preformed on bacteriophage T4 gene 44/62 and 45 proteins. It showed a strong reaction (Mesner et al, 1991; pmid:1720977). The ATPase A activity under either Ca2+-activated or Mg3+-actin-activated conditions were determined in the presence of this antibody. No change in activity was noticed (Eldin et al, 1993; pmid:8448113).
Antibody first published in:
Mesner et al. DNA-dependent adenosinetriphosphatase A is the eukaryotic analogue of the bacteriophage T4 gene 44 protein: immunological identity of DNA replication-associated ATPases Biochemistry. 1991 Dec 10;30(49):11490-4. PMID:1720977
Note on publication:
The construction of three stable murine hybridomas that secrete monoclonal antibodies which recognize calf thymus DNA-dependent adenosinetriphosphatase A is reported. All three of the antibodies react specifically with calf thymus ATPase A and the gene 44 protein from the bacteriophage T4 DNA-dependent ATPase.