United Kingdom (UK)
Recombinant monoclonal antibody to calf thymus ATPaseA. Manufactured using AbAb’s Recombinant Platform with variable regions (i.e. specificity) from the hybridoma 6E12-C1-H2.
UniProt Accession Number of Target Protein: Q9TTA5
Alternative Name(s) of Target: SWI/SNF-related matrix-associated actin-dependent regulator of chromatin subfamily A-like protein 1; HepA-related protein; Sucrose nonfermenting protein 2-like 1
Immunogen: The mouse version of this antibody was generated by immunizing mice with immunopurified calf thymus ATPaseA.
Specificity: This antibody is specific for calf thymus ATPase A and recognizes the c-terminus of native bovine, human, and mouse homologs. Calf ATPaseA is a TP-dependent annealing helicase that binds selectively to fork DNA relative to ssDNA or dsDNA and catalyzes the rewinding of the stably unwound DNA. The antibody shows very little reaction to denatured protein.
Application Notes: The mouse version of this antibody was used for a western blot on calf DNA-dependent ATPase A isolated from calf thymus using immunoprecipitation (Mesner et al, 1993; pmid:8394114). To identify the mouse version of this antibody an ELISA on native ATPase A was preformed. Furthermore, a western blot was preformed on bacteriophage T4 gene 44/62 and 45 proteins. It showed very little reaction (Mesner et al, 1991; pmid:1720977).
Antibody first published in:
Mesner et al. DNA-dependent adenosinetriphosphatase A is the eukaryotic analogue of the bacteriophage T4 gene 44 protein: immunological identity of DNA replication-associated ATPases Biochemistry. 1991 Dec 10;30(49):11490-4. PMID:1720977
Note on publication:
The construction of three stable murine hybridomas that secrete monoclonal antibodies which recognize calf thymus DNA-dependent adenosinetriphosphatase A is reported. All three of the antibodies react specifically with calf thymus ATPase A and the gene 44 protein from the bacteriophage T4 DNA-dependent ATPase.