United Kingdom (UK)
Recombinant monoclonal antibody to Calmodulin-domain protein kinase 1. Manufactured using AbAb’s Recombinant Platform with variable regions (i.e. specificity) from phage display VHH-1B7.
UniProt Accession Number of Target Protein: Q9BJF5
Alternative Name(s) of Target: CDPK1
Immunogen: VHH phage display libraries from alpacas were pooled and panned against recombinant TgCDPK1, and from this clone 1B7 was selected.
Specificity: CDPKs regulate essential processes in parasites.VHH-IB7 recognises CDPK1 in a conformation-dependent manner and selectively inhibits the Ca^2+ bound form. It also recognises CDPK3 in a Ca2+-bound form.
Application Notes: ELISA showed VHH-1B7 has reactivity against CDPK1. By immunoblot, 1B7 recognized both endogenous and recombinant versions of CDPK1 and its closest paralog CDPK3 (Ingram et al, 2015). Immunoprecipitation showed VHH-1B7 specifically recognises the Ca2+-bound form of CDPK1 and CDPK3. VHH-1B7 inhibits CDPK1 and CDPK3 with an IC50 of approximately 40 nm. The crystal structure of CDPK1 with VHH-1B7 showed VHH-1B7 interacts directly with the regulatory domain of CDPK1.
Antibody first published in:
Ingram et al, 2015. Allosteric activation of apicomplexan calcium-dependent protein kinases. Proc Natl Acad Sci U S A. 2015 Sep 8;112(36):E4975-84 PMID:26305940
Note on publication:
Describes the purification of VHH-1B7 from the phage library and its characterisation through ELISA, immunoprecipitation, and crystallography.