United Kingdom (UK)
Recombinant monoclonal antibody to Cap, Capsid protein. Manufactured using AbAb’s Recombinant Platform with variable regions (i.e. specificity) from the hybridoma 3A5.
UniProt Accession Number of Target Protein: O56129; F5A4Z4
Alternative Name(s) of Target: ORF2
Immunogen: The original antibody was raised by immunizing a BALB/c mouse with a recombinant capsid protein of PCV2 expressed by a baculovirus vector
Specificity: This antibody is specific for the epitopes found at the topmost edges around the 5-fold axes of the PCV2, Cap with each epitope covering 18 amino acids from two adjacent Cap proteins.
Application Notes: The original antibody was used for an ELISA on PCV2-positive serum (Huang et al., 2011; PMID:20923690), on PCV2a/LG-infected PK15 cells cultures (Huang et al., 2019; PMID:30863876), and on purified PCV2 (Bian et al., 2021; PMID:34959519). Further this antibody was used for immunohistochemistry on inguinal lymph nodes of PCV2-inoculated pigs (Huang et al., 2016; PMID:27020283) (Huang et al., 2019; PMID:30863876). This antibody was used for an neutralization assay on PCV2a/LG. PCV2a/LG was incubated with this antibody after which the mixture was added to PK15 cells (Huang et al., 2020; PMID:32075932) (Yu et al., 2022; PMID:35945364). This antibody was also used for immunofluorescence to test the absorption of PCV2 to PK15 cells. The structure of the original antibody was determined using cryo-EM (Huang et al., 2020; PMID:32075932). This antibody was used for purification of proteins from PCV2a/LG (Bian et al., 2021; PMID:34959519)
Antibody first published in:
Huang et al. Development of a blocking ELISA for detection of serum neutralizing antibodies against porcine circovirus type 2 J Virol Methods. 2011 Jan;171(1):26-33. PMID:20923690
Note on publication:
The original paper developed a monoclonal antibody (Mab)-based blocking ELISA for the detection of serum neutralizing antibodies to porcine circovirus type 2 (PCV2).