United Kingdom (UK)
Recombinant monoclonal antibody to Capsid protein VP1. Manufactured using AbAb’s Recombinant Platform with variable regions (i.e. specificity) from the hybridoma Mab14.
UniProt Accession Number of Target Protein: P17455
Alternative Name(s) of Target: Coat protein VP1; CPV-2; Fab 14; Antibody 14; ABCD_AD535
Immunogen: The original antibody was generated by immunizing BALB/c mice with purified Capsids of CPV and FPV which were prepared from infected feline cells.
Specificity: This antibody is specific for Site A of CPV-2's capsid.
Application Notes: The original version of this antibody (IgG2a) was raised and tested against CPA, FPV, and MEV and was found to inhibit and neutralize CPV-a and -b in neutralization and HI assays (Parrish et al., 1982; PMID: 6180709). The original version of this antibody was used in RIA and HI assays. In the HI test, it was found to inhibit CPV-a;-b;-c-;-d; and -e, and in the RIA, it was found to react with CPV-d variants 6, 12, and 8 (Parrish and Carmichael, 1983; PMID: 6194613). The Fab version of this antibody was used in a cryoEM analysis of capsid:Fab complexes, and its Kd was measured via ELISA as 3.4 × 10-9 M (Nelson et al., 2007; PMID: 17217977). The Fab version of this antibody was crystallized, and its structure was determined (Hafenstein et al., 2009; PMID: 19321620).
Antibody first published in:
Parrish et al. Antigenic relationships between canine parvovirus type 2, feline panleukopenia virus and mink enteritis virus using conventional antisera and monoclonal antibodies. Arch Virol. 1982;72(4):267-78. doi: 10.1007/BF01315223. PMID:6180709
Note on publication:
The original publication investigates the antigenic relationships between canine parvovirus type 2 (CPV), feline panleukopenia virus (FPV), and mink enteritis virus (MEV) using conventional antisera and monoclonal antibodies.