United Kingdom (UK)
Recombinant monoclonal antibody to Capsid protein VP1. Manufactured using AbAb’s Recombinant Platform with variable regions (i.e. specificity) from the hybridoma MabB.
UniProt Accession Number of Target Protein: P17455; P04864
Alternative Name(s) of Target: Coat protein VP1; CPV-2; FPV; MEV; RPV; ABCD_AD539
Immunogen: The original antibody was generated by immunizing a Lewis-strain rat with purified infectious (heavy) FPV-b.
Specificity: This antibody is specific for CPV-2's, FPV's capsid (site A). It also cross-reacts with MEV and RPV.
Application Notes: The original version of this antibody (rat IgG2b) was raised and tested against CPA, FPV, and MEV and was found to inhibit and neutralize CPV, FPV, and MEV-a and -b in neutralization and HI assays. It was also used in RIAs. In the HI test, it was found to inhibit CPV-a;-b;-c-;-d; and -e, FPV-a; -b; -c, RPV-a; -b; -c; -d; and -e, and MEV-a; -b; -c; -d; -e; -f; -g; and -h. In the RIA, it was found to react with CPV-d and FPV-b (Parrish and Carmichael, 1983; PMID: 6194613). The Fab version of this antibody was used in a cryoEM analysis of capsid:Fab complexes, and its Kd was estimated via ELISA as ~6.1 × 10-9 M (Nelson et al., 2007; PMID: 17217977). The Fab version of this antibody was crystallized, and its structure was determined (Hafenstein et al., 2009; PMID: 19321620).
Antibody first published in:
Parrish & Carmichael Antigenic structure and variation of canine parvovirus type-2, feline panleukopenia virus, and mink enteritis virus. Virology. 1983 Sep;129(2):401-14. doi: 10.1016/0042-6822(83)90179-4. PMID:6194613
Note on publication:
The original publication investigates the antigenic structure and variation of canine parvovirus type-2, feline panleukopenia virus, and mink enteritis virus, employing monoclonal antibodies to identify antigenic variants within and between virus isolates from different animal species.